FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51400977
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT611950.11905415727798326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC144550.022.6176074
GACCGCG150150.021.7590545
ACCGCGT152850.021.397616
CAGACCG155600.021.1996563
CCGCGTT166050.019.710177
CGCGTTC159850.019.5600018
CCGTACG65500.016.236714
TCAGACC207800.016.1672
GAACGAT204350.015.61926445
CGTACGC69050.015.4998425
GCGTTCT202650.015.4843979
CTCAGAC255800.015.3168231
CCCGTAT34650.014.386611
GTCCGTT40000.014.3233021
CTCCGAT48850.014.3141821
TACGCCA76800.014.0294487
ATACGGA233650.013.90185533
TACGGAG235200.013.72405734
AATACGG237850.013.58079532
AGAACGA237150.013.49733544
Produced by FastQC (version 0.11.5)