FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51400977
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG154950.012.08345345
GGCATAG157200.011.72189935
ATAGGGG163050.011.65982438
GGGAGGT244450.011.5915683
GTTGTCG24300.011.19882326
GGAGGTC254850.010.586624
GAGCTTA181400.010.3580344
TCAGACG97700.010.2593742
TGGCATA183450.010.17951434
GGTCACT267050.09.8513857
CATAGGG192950.09.8412937
GCATAGG199450.09.48685536
GTCACTC287450.09.2302838
ACTCTCC289600.09.12271711
AGGGATC139050.08.7635832
CAGACGT114800.08.5741523
TAGGGGA304000.08.55557539
CGTGGCG147100.08.4105731
AGGGGAG330700.08.13612440
TGGGAGG256650.08.1093512
Produced by FastQC (version 0.11.5)