FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49423148
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1110960.22478535766276966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT210700.024.58958845
AGACCGC240000.022.1660984
GACCGCG245700.021.587785
CAGACCG246950.021.5605743
AGAACGA250950.020.80729744
ACCGCGT255700.020.7691546
CCGCGTT261000.020.3386427
CGCGTTC266100.019.9403848
AATACGG287600.018.3122332
TACGGAG287800.018.23807534
GCGTTCT292550.018.175989
ATACGGA290250.018.1457933
TCGGCGC110300.018.00019645
TTCGGCG110800.017.95964444
AAGAACG290950.017.93820443
CCGTACG116850.017.5756514
CGTACGC118800.017.287165
CAATACG310500.017.12006231
ACGGAGA313050.016.78928435
CGGAGAG320650.016.32185636
Produced by FastQC (version 0.11.5)