FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49423148
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG86250.010.3222942
GGCATAG106300.010.24682835
GCGTCGT24950.010.08799311
CATAGGG107750.010.07633737
GTCACTC160500.09.8837528
CGTTAAT27550.09.8742461
CGTGGCG135150.09.8645851
TAATCGG57600.09.6398174
AGCTTAG116950.09.55990745
GCATAGG114600.09.472239536
ATAGGGG124000.09.24134538
GGGAGGT193450.08.9834283
TCACCCG78950.08.9414693
CGACCCG147700.08.8958586
GGTCACT177900.08.8655427
ACCCGGC80700.08.8010465
GAGCTTA124200.08.79256544
ACTCTCC188100.08.745398511
TCGTGTA29250.08.68544914
GCGACCC154700.08.6737085
Produced by FastQC (version 0.11.5)