FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45895040
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT989860.2156790799180042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT181600.026.54676445
AGACCGC215850.022.0743224
AGAACGA222600.021.66759344
CAGACCG226100.021.2228073
GACCGCG233550.020.362855
ACCGCGT236950.020.1561786
TTCGGCG67550.020.09118744
CCGCGTT243850.019.5766757
CGCGTTC246550.019.3805378
AATACGG251300.019.0119532
ATACGGA250900.019.00704633
TACGGAG252400.018.94826934
AAGAACG264400.018.19951843
GCGTTCT266900.017.9787149
TTCGACG19150.017.738756
CAATACG271550.017.69624131
ACGGAGA274650.017.51211435
TCAGACC280500.017.4918332
GTCCGTT39300.017.1853681
CGGAGAG287350.016.63630736
Produced by FastQC (version 0.11.5)