FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48273397
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1275600.2642449214833586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT254350.023.75986145
AGACCGC276650.022.0809754
CAGACCG284400.021.5512223
GACCGCG302200.020.2066175
AGAACGA307950.019.8654644
ACCGCGT310650.019.6710746
CGCGTTC317550.019.2578148
CCGCGTT317950.019.2265117
GCGTTCT336850.018.214549
TCAGACC341900.018.1637022
CTCAGAC372300.017.8054521
TTCGACG17200.017.7901696
AAGAACG349950.017.5069843
AATACGG348700.017.47940832
ATACGGA354850.017.17012633
TACGGAG355950.017.0791434
CAATACG367900.016.58553931
CGTTACG20150.016.41553921
ACGGAGA379250.016.07137735
CGGAGAG385750.015.765573536
Produced by FastQC (version 0.11.5)