FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48273397
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG594420.12313614473827064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG208550.014.70721345
GGGAGGT328550.014.5975343
ATAGGGG217350.014.19046138
GGCATAG219100.013.92243935
GGTCACT333900.013.8689787
GTCACTC343750.013.60862358
GGAGGTC351300.013.5621064
ACTCTCC362350.012.91634811
GAGCTTA237100.012.86961744
TGGCATA245000.012.59739234
AGGTCAC386000.012.3703146
GAGGTCA389800.012.238395
TCACTCT393350.012.075749
CACTCTC393350.011.83548410
GTTGTCG26800.011.83494326
CATAGGG271550.011.49070637
TGGGAGG300500.011.0372522
GCATAGG288500.010.72170536
GCTCTGT436300.010.66306922
AGCATAG149650.010.405766545
Produced by FastQC (version 0.11.5)