FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46514049
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG111900.012.76753145
GGCATAG113300.012.37164735
ACCCGGC81300.011.6507515
ATAGGGG123000.011.59774638
GAGCTTA127150.011.32468144
CATAGGG128850.011.01881437
GTTGTCG17650.010.963526
GGGAGGT226600.010.6644973
TCACCCG95700.010.6037783
TGGCATA135400.010.51853734
GCATAGG135300.010.44361436
ATAGCTC87100.010.436339
ACACGGA95750.09.96278116
CCGGACA106500.09.80196212
ACTCTCC219650.09.72018411
TCAGACG74550.09.63184452
TAGGCTA78450.09.54972310
AGGGATC128000.09.5489652
TAGCTCT95600.09.53191240
GGTCACT217900.09.4783947
Produced by FastQC (version 0.11.5)