FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52715075
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4321550.8197939583695935No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1018770.19325970796778721No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG950210.18025394064221667No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC583250.11064197480511978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT704050.031.61192945
AGAACGA833950.026.75803644
AGACCGC874200.025.542974
GACCGCG879100.025.2956625
CAGACCG889650.025.1878593
ACCGCGT885350.025.1297916
CCGCGTT896400.024.8300517
CGCGTTC901900.024.5888638
TCAGACC936700.024.2472442
AAGAACG939350.023.72688343
CTCAGAC984800.023.7089881
GCGTTCT943350.023.5538799
AATACGG971900.022.86201732
TACGGAG970000.022.74905234
ATACGGA977650.022.66084533
CAATACG997300.022.40377631
ACGGAGA1001950.022.01694335
CGTTCTC1029500.021.92145510
CGGAGAG1005750.021.78917736
ACTCCCC1035500.021.73380925
Produced by FastQC (version 0.11.5)