FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52715075
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA818340.1552383260386142No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG768730.14582735583701625No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG749860.1422477346375776No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT695160.1318711962375089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG181850.013.93021345
ATAGGGG192450.013.0486538
GGCATAG193950.012.78331435
GTCACTC299200.012.2442418
GGGAGGT317150.012.0913313
GGTCACT306200.012.0812597
GTTGTCG25500.011.64460526
TGGCATA220400.011.47377534
GAGCTTA221800.011.46372844
GGAGGTC337200.011.3051214
AGGGATC187950.011.0400392
CATAGGG229700.010.93178737
TCACTCT346850.010.8605239
ACTCTCC342450.010.69127511
GCATAGG239900.010.42887136
TAGGCTA80850.010.18685510
AAGCCGG364250.010.11071245
TAGCGTA4905.456968E-1210.10216637
AGGTCAC380450.010.1018976
AGCATAG114200.09.94850745
Produced by FastQC (version 0.11.5)