FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51895358
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG131350.014.04546345
GGCATAG141750.012.97094235
ATAGGGG145550.012.90987438
GTTGTCG27100.012.53609326
ATATAGC29350.012.4195766
GAGCTTA155900.011.80133844
GGGAGGT265700.011.6787653
GCCACGA163550.010.85549932
CATAGGG178900.010.37805837
CACGAAG174500.010.21313334
GACGCAA35200.010.1720844
ACATGCG149000.010.0692610
TGGCATA186400.09.99571134
CCACGAA177850.09.944756533
CAATATA43250.09.93742754
AGCATAG92550.09.83302545
AGCCACG183050.09.8220631
GCATAGG189000.09.79964936
TCGATAA37000.09.72550116
ACTCTCC292000.09.69690811
Produced by FastQC (version 0.11.5)