FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49893431
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3903500.7823675224900848No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA789690.1582753449046228No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG733250.14696323449874593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT588200.032.04205745
AGAACGA682300.027.6757244
AGACCGC701700.027.238014
CAGACCG711500.026.9103033
GACCGCG711700.026.87115
ACCGCGT724400.026.4155316
CCGCGTT733850.026.065817
CGCGTTC735700.025.9849728
TCAGACC765900.025.1473982
GCGTTCT767600.024.9578469
AAGAACG756250.024.9424943
CTCAGAC796500.024.7501351
AATACGG780400.024.14308232
TACGGAG781700.024.0702334
ATACGGA784350.024.0329733
CAATACG797200.023.67945331
CCCAATA808550.023.32200829
ACGGAGA811300.023.2337335
CGGAGAG819400.022.96312336
ACTCCCC823700.022.88431225
Produced by FastQC (version 0.11.5)