FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49893431
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG596110.1194766501425809No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG563340.11290865124108222No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG514310.10308170628714629No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT508120.10184106200273138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG193200.015.32315345
ATAGGGG195250.015.05004438
GTTGTCG25900.015.02689526
GGCATAG204200.014.37836535
GGGAGGT304800.014.0580413
GGTCACT294550.014.0568357
GTCACTC300850.013.7922858
GAGCTTA220900.013.50488144
TGGCATA232900.012.71214734
ACTCTCC334300.012.61457111
GGAGGTC341150.012.5986174
AGGTCAC354750.012.00723656
AGGGATC176950.011.9181512
CATAGGG250600.011.91409637
GAGGTCA368150.011.7965435
TCACTCT367000.011.5825059
CACTCTC366000.011.39896210
GCATAGG264050.011.2812236
TGTCGTT36350.011.01613628
TGGGAGG292200.010.7451852
Produced by FastQC (version 0.11.5)