FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47787418
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT855630.17904922170099252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT176100.023.3006245
TTCGGCG107800.021.1858644
AGACCGC196150.020.838274
CAGACCG206150.019.980213
AGAACGA217950.018.85754844
TCGGCGC121900.018.64284745
GACCGCG225350.018.0183375
CCGTACG126950.017.8971444
CGTACGC129650.017.5244315
ACCGCGT243050.016.8355436
TACGCCA136550.016.8028897
TACGGAG245400.016.61123334
ATACGGA247950.016.49452633
TCCGTAC143150.016.3431993
CCGCGTT250350.016.317137
GTACGCC140600.016.3034296
AATACGG251250.016.19655232
GCGTTCT254700.016.0560829
CCCGTAT30650.016.0068571
CGCGTTC255250.016.0038938
Produced by FastQC (version 0.11.5)