FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50686627
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT757870.14952070099278847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27850.023.4260046
GAACGAT183200.020.5810345
AGACCGC183100.020.4451564
CAGACCG190100.019.8461553
GACCGCG207100.018.0975725
CGGTATA47200.017.96781320
TCGACGA38350.017.4802327
ACCGCGT222150.017.0031686
CCGCGTT229350.016.4977057
CGACGAG39950.016.3296598
CGCGTTC234150.016.1787078
GTCCGTT42650.016.170191
ATACGGA234650.015.9194133
AATACGG235350.015.83325232
CGTTACG14000.015.74685921
TACGGAG237000.015.71383634
AGAACGA241050.015.61669944
CTCAGAC274050.015.4693381
TCAGACC250350.015.3484922
GCGTTCT254000.014.9763429
Produced by FastQC (version 0.11.5)