FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50874343
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1302650.25605244671169514No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG934330.18365446016668951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC261900.024.8715114
GACCGCG266700.024.2382535
ACCGCGT267550.024.135766
CAGACCG271250.024.0889873
CGCGTTC286350.022.5504658
CCGCGTT288500.022.3906277
TCAGACC333300.020.010842
GCGTTCT328800.019.7821069
GAACGAT347700.018.44245145
CTCAGAC407700.017.6624151
CGTTCTC400500.016.65629810
CTACACG137400.016.42451945
AGAACGA393800.016.3632144
CCGGCTT352300.016.2617842
TCACGCT334450.016.212758
CCCGTAT41450.016.0204261
AATACGG401800.015.9093732
TTCACGC342250.015.81067857
TACGGAG405050.015.75403634
ACTCCCC415950.015.72967725
Produced by FastQC (version 0.11.5)