FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44927178
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG20350.013.16915326
AGCTTAG157000.012.95425545
GGCATAG159100.012.6636135
GGGAGGT228450.012.6404473
ATAGGGG164300.012.63731538
GAGCTTA173450.011.83254844
GGAGGTC236750.011.8271074
TGGCATA175000.011.67915334
TCAGACG98400.011.6703932
GGTCACT245100.011.2080847
CATAGGG188700.010.98193537
ACTCTCC259600.010.631165511
GCATAGG197000.010.46056836
GTCACTC273950.010.1276278
GAGGTCA291650.09.9621845
CAGACGT114750.09.9094213
ATCCTAG110350.09.3225266
TAGGCTA91700.09.26304110
TATCCTA97800.09.2594295
CGTAACG13200.09.22402942
Produced by FastQC (version 0.11.5)