FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44595372
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC102750.017.9866925
ACGGACA116450.015.45589818
TCACCCG130200.015.0953793
CACCCGG126050.014.8943474
CGGACAC121150.014.80057813
CCGGACA128450.014.2396812
CGGACAG127850.014.165729519
CACGGAC126900.014.07675817
ACACGGA130300.013.74410116
ATAGCTC128950.013.55778539
GCCCGGA136250.013.4905410
GACACGG138900.013.07143715
TAGCGTA9350.012.75423237
AGCTTAG119950.012.30524445
GGCATAG118450.012.28996135
TAGCTCT144900.012.18959840
CGGCCCG164950.011.7018068
TGATAGC155900.011.40173837
GGATTGA161350.011.3493125
TTGATAG160300.011.18740836
Produced by FastQC (version 0.11.5)