FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50466102
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG532820.10557978105778806No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG524730.1039767248122314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28800.014.29581526
AGCTTAG192150.013.86977345
ATAGGGG202550.013.13952238
GGGAGGT302850.013.0575253
GGCATAG203250.012.90530735
GGAGGTC309300.012.7122854
GAGCTTA224050.011.98722844
TGGCATA226300.011.65050334
ACTCTCC347200.011.16137111
TGTCGTT38250.011.05709328
CATAGGG247200.010.77520637
CACTCTC365300.010.54021210
GCATAGG262400.010.1764836
GTCGTTC41600.010.16663729
GTCACTC387800.010.0510828
GGTCACT385850.010.0202127
TAGGGGA386400.09.82202839
TCTCCCC397550.09.72553713
TGGGAGG303350.09.5566432
TGTCCAA407150.09.54307226
Produced by FastQC (version 0.11.5)