FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47955339
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT754330.157298439700322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG51450.025.5798326
CGGTATA70100.025.04723420
TCGACGA60800.022.1270927
CGACGAG58350.021.8972428
GTTCGAC58750.021.4851935
AGACCGC179150.020.4340784
GACCGCG182250.020.0371195
ACCGCGT184050.019.911896
CAGACCG187000.019.8048923
CCGCGTT187500.019.5214947
CGCGTTC188500.019.4159268
GAACGAT185600.019.04236645
AGTTCGA73550.017.9266514
GTATAGA108850.017.17327522
ATAGAGT108500.017.0007624
TCAGACC225150.016.6989352
AACGACG33200.016.001616
ATGACGG103200.015.98872416
GACGAGG104850.015.919069
ACGAGGA105150.015.78831610
Produced by FastQC (version 0.11.5)