FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48163908
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1233330.25606933723069153No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1131020.23482729017753295No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG711100.14764167392729013No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG611670.12699758499663275No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA522250.10843181579036319No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACTTCTCTTCCTCGTGGTTTTCG510360.10596316229156488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG265200.021.1676395
AGACCGC267400.021.086044
ACCGCGT269200.020.8944456
GCCGGCT420750.020.83441
CAGACCG273150.020.8151443
CCGGCTT502350.020.1464422
CCGCGTT296350.018.9953927
ACACCGG74300.018.80671326
CGCGTTC300450.018.7811098
CGGCTTC550350.018.77323
TTCACGC532350.018.7439987
TCACGCT537750.018.4888328
TCAGACC321200.018.1430192
ACCGGTG76600.018.0361528
CGTTACG22800.017.56814421
CACCGGT80800.017.23804527
AACACCG81000.017.13997725
ACGCTCA594550.017.06309310
CTCAGAC376550.016.7548891
GTCCGTT44450.016.6536851
Produced by FastQC (version 0.11.5)