FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48163908
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1199780.24910354035224883No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1119840.23250604996587904No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG850700.17662603292075055No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA545810.11332344543137987No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG541920.11251578671730707No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG528820.10979590775731903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC395900.014.9750014
GTTGTCG39300.014.93909426
GGGAGGT400900.014.9341293
AGCTTAG307700.013.6549145
GAGGTCA445250.013.54772955
GGTCACT434600.013.4724977
AGGTCAC450450.013.3281486
ATAGGGG330400.012.77627938
GTCACTC471950.012.559458
GGCATAG332650.012.55853635
AGGGATC229800.012.3707692
TGGGAGG358500.012.1927992
ACTCTCC486050.012.12988811
ATATAGC57200.012.0773856
TCACTCT496650.011.9982639
TGGCATA350400.011.97375234
CACTCTC492900.011.94344910
GAGCTTA355650.011.77595344
TGTCGTT52250.011.62382928
CTGGGAG405450.011.58628751
Produced by FastQC (version 0.11.5)