FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51213629
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG889850.17375257668227337No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT566180.11055260309711698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30300.021.162566
CGTTACG27550.019.11016521
TCGACGA36050.018.598467
CGGTATA46700.017.39243920
GCCGGCT291900.017.1796671
CCGGCTT351200.016.491312
GTTCGAC38450.016.3855085
TCGGCGC128650.015.79264745
TCACGCT366550.015.2163058
TTCGGCG134750.015.21130844
TTCACGC370550.015.0702667
GTCCGTT38050.015.0316851
CGACGAG42150.014.9460498
GTCCGAT25700.014.8951341
GTTACGA37450.014.59905122
CGGCTTC416750.014.52901653
AACGCTA370900.014.47994923
ACGCTAC374500.014.38881924
CCCGTAT36700.014.2961281
AGACCGC122950.014.2929114
Produced by FastQC (version 0.11.5)