FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51213629
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA765920.14955394002639416No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG606860.11849580118604755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG171850.012.74259845
GGCATAG183300.011.83403435
ATAGGGG191650.011.50689138
GTCACTC247700.011.4430838
GGGAGGT266600.011.0118193
TCAGACG133850.010.9917912
GAGCTTA200050.010.99134144
CACCGAA374800.010.95266845
GGTCACT259700.010.9139787
CGTGGCG224350.010.7538891
ACTCTCC275200.010.292870511
TATCCTA136450.010.28770355
CCGTACG14450.010.2750394
GGAGGTC293950.010.124974
GCACCGA406750.010.09234244
TCCTAGG140250.010.0565537
TGGCATA215350.010.03117634
CCTAGGC137350.09.9088958
GTTGTCG22500.09.89960526
CATAGGG220400.09.8628537
Produced by FastQC (version 0.11.5)