FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49494989
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG133650.012.56550438
AGCTTAG139950.011.93016445
GGCATAG139500.011.86835335
GGGAGGT223600.011.0979473
CATAGGG154350.010.78802237
GGTCACT221800.010.7574627
GGAGGTC231400.010.7432864
GTCACTC230600.010.6609568
GCATAGG159550.010.54877236
GAGCTTA162400.010.47730944
ACTCTCC241550.010.43069511
TGGCATA164100.010.20362234
GTTGTCG21900.09.97512826
AGGTCAC257900.09.6960746
AAGCCGG178400.09.64899445
CGTAGGG73550.09.44844137
TAGGGGA250750.09.44165939
GAGGTCA274000.09.3827355
GCGTAGG73950.09.27460236
GGCGTAG75700.09.20727735
Produced by FastQC (version 0.11.5)