FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46255656
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT669850.14481472276601157No Hit
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC559930.1210511423727295No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG518740.11214628541858752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT128200.024.76098845
ATACGGG148250.021.43122133
AGACCGC148950.021.402154
AATACGG151200.021.05943932
ACCGCGT151700.020.9103646
TACGGGG154600.020.59529334
CGCGTTC155300.020.4111568
CCGCGTT158150.020.0860047
CAGACCG161800.019.8692633
AGAACGA162900.019.54179244
GACCGCG165700.019.1843765
CAATACG168200.019.03815331
ACGGGGA181050.017.63644235
AAGAACG181050.017.59519843
AACGCTA88850.017.55106223
TTCGACG18800.017.4712126
ACGCTAC92400.016.82806424
TCAGACC194600.016.820862
CGCTACC97100.015.99074525
CCCAATA202100.015.97838629
Produced by FastQC (version 0.11.5)