FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47006338
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG532740.11333365300653711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG163250.016.07524745
ATAGGGG165500.015.71880338
GGCATAG180300.014.46540835
GAGCTTA191600.013.77884944
TGGCATA193550.013.52158734
GGGAGGT280750.013.4618713
GGTCACT272650.013.3000077
GTCACTC287550.012.8142958
CATAGGG209700.012.56651537
AGGTCAC303750.012.4640126
GGAGGTC306350.012.4543124
ACTCTCC305000.012.35517611
GAGGTCA326600.011.7509325
GCATAGG226500.011.7139536
GTTGTCG20000.011.58701726
TCACTCT338350.011.0500239
CACTCTC336550.011.04896510
TAGGGGA328400.011.04644239
TGGGAGG278650.010.2209342
AGGGGAG374650.09.89396740
Produced by FastQC (version 0.11.5)