FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51480301
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT688710.1337812690722224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG48050.026.8787786
TCGACGA55850.023.1648227
GTTCGAC54400.023.1622225
CGGTATA78550.022.6564720
CGACGAG57200.022.105948
GACCGCG162150.020.6618295
ACCGCGT164950.020.31116
AGACCGC168800.019.927864
CCGCGTT175350.019.1318157
GAACGAT175200.019.12325345
CGCGTTC177900.018.8442578
CAGACCG180300.018.7568283
AGTTCGA73250.018.000394
GCCGGCT186750.017.9088271
CGTTACG26800.017.54584521
ATGACGG98500.017.35924716
CCGGCTT220850.017.055082
GACGAGG100750.016.8374399
ACGAGGA100500.016.76743910
AACGACG35950.016.58442916
Produced by FastQC (version 0.11.5)