FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51480301
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG184800.013.4784438
AGCTTAG191200.013.0426745
AACTCTA119400.012.98048445
GGCATAG191200.012.82708535
GGGAGGT273450.012.7879673
GTTGTCG26700.012.38942626
GGAGGTC278350.012.2656094
GAGCTTA209550.011.8154644
GTCACTC289000.011.6693168
TGGCATA222350.011.1825934
ACTCTCC306000.011.14044511
GGTCACT301650.011.0449937
CATAGGG230250.010.798141537
AGGTCAC327400.010.6497786
GCATAGG242950.010.1689236
GAGGTCA350400.09.9761325
TATATAC77100.09.8622517
TAATCGG51600.09.6763564
ATATATA85350.09.6200416
CACTCTC350200.09.55933210
Produced by FastQC (version 0.11.5)