FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45654887
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG23700.012.53158326
GGGAGGT202350.012.1756113
ATAGGGG156450.011.89367238
GGCATAG155000.011.671061535
AGCTTAG160550.011.40759145
GGAGGTC213800.011.386744
GTCACTC232150.010.5642258
GAGCTTA178000.010.28926244
GGTCACT235550.010.2302487
TGGCATA182950.010.07274834
CATAGGG185450.010.02164937
GAGGTCA252750.09.9702645
ACTCTCC248350.09.77545811
GCATAGG195850.09.36310836
TGTCGTT35450.09.3302128
CGTAACG13300.08.9661142
TCACTCT276900.08.9544419
CACTCTC266300.08.8630710
ATATAGC42500.08.8411226
AGGGATC123800.08.7600432
Produced by FastQC (version 0.11.5)