FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48919355
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2314130.47304998195499515No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA856020.1749859539235544No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG844740.17268011812502435No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC557570.11397738175411348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT398150.028.24434745
TTCGACG25950.024.4464446
AGAACGA515000.021.8790444
GACCGCG555300.020.1548715
ACCGCGT558350.020.052346
AGACCGC562000.019.9876654
CCGCGTT573450.019.5516077
CGCGTTC573650.019.5323858
CAGACCG579450.019.4245683
TCAGACC612200.018.6941282
AAGAACG605800.018.5774343
GCGTTCT618550.018.1945579
CGGTATA49300.018.07344820
CTCAGAC664400.018.0354331
TCGACGA35450.018.0219617
GTTCCTA102900.017.5106415
AATACGG644850.017.32670232
ATACGGA649250.017.15552533
TACGGAG648600.017.08502634
CGTTCTC681900.016.78129410
Produced by FastQC (version 0.11.5)