FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48919355
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG624530.12766521553687696No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA597850.12221134150276511No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG596210.12187609587248237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33600.016.53904326
AGCTTAG216200.014.13878945
GGGAGGT301650.013.9795263
GGAGGTC300300.013.6228614
ATAGGGG229000.013.5903838
GGCATAG226350.013.56133335
GAGCTTA236750.012.84120144
TGGCATA249750.012.34368534
GGTCACT342550.011.8760667
ACTCTCC347500.011.83052311
CATAGGG262900.011.77867337
GTCACTC351600.011.6974018
TGTCGTT47300.011.65298428
GCTAAGT49500.011.585525514
GAGGTCA366250.011.4517945
GCATAGG277950.011.15709436
CACTCTC369000.010.98451510
TGGGAGG308450.010.541842
TCGTTCC52700.010.50292230
ATATAGC59350.010.3880546
Produced by FastQC (version 0.11.5)