FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48902996
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT767970.15703945827777094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT207200.018.24261745
TTCGGCG74800.016.96469344
AGACCGC225100.016.5633414
CAGACCG233200.016.0941643
GTCCGTT36550.016.0163351
TTCGACG14050.015.6934116
GACCGCG239800.015.5292235
ACCGCGT248900.015.0507096
AGAACGA253750.014.88720444
CCGCGTT259150.014.4640997
CCGTACG88950.014.4188234
CGCGTTC262100.014.3270558
TCGGCGC90400.014.31090345
CGTACGC90450.014.2294585
CCCGTAT32300.013.66251851
TCAGACC285800.013.36831952
TACGCCA97700.013.287677
TACGGTA78450.013.250051544
ATACGGA285400.013.18146533
CCCCCCT45500.013.1627741
Produced by FastQC (version 0.11.5)