FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48902996
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG133850.012.5776345
ACCCGGC78500.011.4918215
GGCATAG148800.011.31184135
ATAGGGG152050.011.30711538
GAGCTTA162400.010.602077544
GGGAGGT261150.010.3028633
ATAGCTC81000.010.16762639
CATAGGG171200.09.97663837
TGGCATA171250.09.86816634
GTCACTC256350.09.8112928
ACTCTCC255250.09.69508711
GCATAGG180000.09.45113536
GGAGGTC284700.09.1265734
TCACCCG107050.09.0784953
TAGCTCT91300.09.0697140
ACACGGA98700.08.86709316
ACGGACA101950.08.827641518
CACGGAC103550.08.734617
CGGACAG102500.08.71442119
GAGGTCA300550.08.6452875
Produced by FastQC (version 0.11.5)