FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53345360
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG174700.013.61405245
GGGAGGT267900.012.9572963
GGCATAG182450.012.90321535
ATAGGGG186450.012.7833238
GAGCTTA195200.011.98601244
GGAGGTC280250.011.6798854
TGGCATA205100.011.48923634
ACCCGGC76900.011.3508025
CATAGGG211200.011.20002337
GCATAGG221450.010.6816236
ATAGCTC77400.010.46819239
GTTGTCG23200.010.375689526
ACTCTCC317800.010.32815211
TCACCCG93000.09.9179543
GGTCACT324500.09.8583927
TCAGACG90600.09.8578792
TGGGAGG266950.09.3796452
GTCACTC353950.09.203398
GCTCTGT355500.09.17591922
TAGGGGA347150.09.16731939
Produced by FastQC (version 0.11.5)