FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45656344
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT785670.1720834239377555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT186250.020.95855945
AGACCGC193100.019.9387654
CAGACCG207500.018.7177313
GACCGCG211500.018.1403435
ACCGCGT213950.017.950546
CCGCGTT220800.017.4242027
AGAACGA224500.017.32753844
TTCGACG15600.017.1624266
CGCGTTC227150.016.9371078
CCGTACG44100.016.5834664
CGTACGC45250.016.2615015
TCAGACC246200.016.1960122
CCCGTAT26050.015.905161
AATACGG244400.015.7334132
ATACGGA246250.015.67016933
AAGAACG250100.015.62604543
CGTTACG16800.015.53484521
TACGGAG252250.015.235702534
CAATACG257450.015.08446531
GCGTTCT257600.014.9786999
Produced by FastQC (version 0.11.5)