FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45656344
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG118550.012.699938
AGCTTAG119000.012.55979745
GGCATAG125500.011.907186535
GGGAGGT201100.011.2943483
GAGCTTA140050.010.81666744
GTTGTCG14700.010.71492826
TGGCATA147000.010.34927434
CATAGGG148750.010.15190237
GGAGGTC231400.09.9236374
CGTGGCG72400.09.4445071
GCATAGG162000.09.40508536
GTCAGGA141800.08.8829261
TAGGGGA242900.08.72788639
CGACCCG72550.08.6808256
GTCACTC269500.08.3882638
GGTCACT261700.08.1825087
ACTCTCC275550.08.09897911
TGGCGAC81550.07.96940233
CGTAGGG79550.07.94965137
GCGTTCG19050.07.912540415
Produced by FastQC (version 0.11.5)