FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48640687
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG149500.012.82801445
ATAGGGG153400.012.48509938
GGCATAG152850.012.42644435
GTTGTCG23050.012.3916126
GGGAGGT239950.012.1703823
GAGCTTA170200.011.17581944
GGTCACT251550.011.1030187
TGGCATA172650.010.9753334
GTCACTC258200.010.9136238
GGAGGTC268150.010.79618554
AGGGATC153550.010.4425242
CATAGGG184700.010.38133737
TCAGACG90700.09.8821432
ACTCTCC287400.09.82041511
TCACTCT303050.09.6331239
GCATAGG199150.09.60543436
CAGGGAT200300.09.1524281
TGTATCC103050.09.0237493
TAGGCTA86250.09.00320110
TGTCGTT33400.08.9579728
Produced by FastQC (version 0.11.5)