FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52007867
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT564040.10845282310847318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC140200.018.8249784
CAGACCG144500.018.4360683
GACCGCG144900.018.1056375
ACCGCGT148250.017.7267066
CCGCGTT158700.016.5877387
TTCGACG17800.016.4324866
GAACGAT164150.016.1739345
CGCGTTC163400.016.138128
GTCCGTT46450.015.88894751
CCCGTAT40200.015.7284981
CCGGCTT237200.015.5660492
TTCACGC202400.014.8182827
ACGCTCA209200.014.74528410
CGTTACG19050.014.52846321
TCACGCT211700.014.1035188
AACGCTA202100.013.93937823
TCAGACC200300.013.7831372
ACGCTAC206400.013.65971824
CGCTACC210450.013.37511325
GCGGACT61200.013.3088832
Produced by FastQC (version 0.11.5)