FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52007867
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24950.013.34264426
AGCTTAG186050.012.9424445
GGGAGGT265450.012.881943
ATAGGGG190300.012.75691738
GGCATAG196550.012.28064735
GGAGGTC295400.011.5678784
GTCACTC299450.011.0626038
GAGCTTA215850.011.04093844
TGGCATA224200.010.8561834
GGTCACT314500.010.6032217
CATAGGG248350.09.93704137
CGCTCGC29150.09.87700125
TGGGAGG273550.09.5627312
GCATAGG259400.09.50486736
TCACTCT378650.09.2122889
GCTAAGT40600.09.20140514
AGGGATC126200.08.9726592
TCGCTCG33450.08.9439924
ATATAGC46050.08.8932666
TAGGGGA376100.08.73406339
Produced by FastQC (version 0.11.5)