FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56554357
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT278900.012.6923233
AGCTTAG188400.012.56370745
ATAGGGG195900.012.40348938
GGCATAG201850.011.93755135
GGAGGTC303800.011.5187014
GAGCTTA221950.010.8973644
ACTCTCC321500.010.72130611
GTTGTCG28150.010.63029926
TGGCATA231850.010.54816734
CATAGGG235700.010.22314337
GGTCACT330000.010.2085617
AGGGATC180750.09.9852672
GTCACTC345950.09.8354718
GCATAGG248000.09.75239636
CACTCTC366550.09.29975710
GAGGTCA396750.09.0810275
TGGGAGG285550.09.0631522
GCTCTGT379150.09.00812522
TAGGGGA372500.08.99338139
GCGTAGG104900.08.90076136
Produced by FastQC (version 0.11.5)