FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49440838
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT130000.024.5084145
AGACCGC157600.020.4045894
AATACGG163400.019.92472532
ATACGGG124800.019.79412833
AGAACGA165950.019.40241644
CAGACCG167500.019.2522893
CCGCGTT170700.018.8103587
GACCGCG170450.018.759695
AACGCTA122550.018.6527623
ACGCTAC123300.018.53909324
ACCGCGT174650.018.4113886
TACGGGG134700.018.3727534
CGCGTTC174700.018.3666958
TTCGACG16750.017.732996
CAATACG186300.017.65673631
CGCTACC131650.017.39729525
AAAACGC143950.016.19228621
CGTACGC62150.016.075725
AAGAACG201450.016.00559843
CCGTACG61850.015.899874
Produced by FastQC (version 0.11.5)