FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49440838
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG120800.010.65099445
GGCATAG119800.010.61169235
ATAGGGG128000.010.17671338
ACCCGGC73300.010.0028975
GGGAGGT232250.09.7500593
GTTGTCG21350.09.69862526
TGGCATA141900.09.1968434
ACTCTCC222700.09.18662411
GAGCTTA143050.09.10472244
CATAGGG141750.09.07971437
GGAGGTC237950.08.9780584
GGTCACT218550.08.8342747
CACTCGA90000.08.7715615
GTCACTC227850.08.68138
GCATAGG148100.08.56894136
ACACTCG97100.08.47634
CGTAGGG46950.08.43482937
ATAGCTC77250.08.328855539
GGCGTAG47100.08.2167635
TAGGGGA239400.08.19426739
Produced by FastQC (version 0.11.5)