FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50682371
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1126100.22218771098929052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC277400.019.3985274
GAACGAT282400.018.91050345
CAGACCG291950.018.601283
GACCGCG294900.018.2473585
ACCGCGT307950.017.5881276
CCGCGTT317450.017.05477
TTCGACG20250.016.8834766
CGCGTTC320950.016.854758
GCGTTCT336900.016.1168779
TCAGACC344100.015.9913762
AGAACGA344750.015.52959644
CTCAGAC384800.015.3990781
GTCCGTT39950.014.9788661
CGTTACG17600.014.70059121
GTTCCTA73900.014.29313115
AATACGG381500.014.11837832
TACGGAG373850.014.08241534
AAGAACG380800.014.06534543
ATACGGA376300.014.05641233
TTCGGCG59350.013.60696344
Produced by FastQC (version 0.11.5)