FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41065218
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG613330.14935510630918847No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA585050.14246849974107043No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG526650.1282472188507559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187650.015.90973445
GGGAGGT247500.015.5293653
GGTCACT252300.014.7757137
GGCATAG201700.014.71279935
ATAGGGG207900.014.49047138
GTCACTC263100.014.2972258
GAGCTTA211250.014.21761244
ACTCTCC275450.013.68925211
TCAGACG111650.013.4104092
GGAGGTC291250.013.2881634
TGGCATA226950.013.17501834
AGGGATC159450.013.1039612
TGGGAGG239900.013.0737442
AGGTCAC297200.012.8766516
CATAGGG237800.012.59280937
GAGGTCA309000.012.5751465
TCACTCT305600.012.5520699
CACTCTC301500.012.43923210
GCATAGG247450.012.07448536
GTTGTCG16750.011.95575626
Produced by FastQC (version 0.11.5)