FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52924951
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG121500.014.41218845
ATAGGGG127350.013.78184238
GGCATAG135400.012.94584635
CATAGGG150700.011.85547337
GAGCTTA150200.011.67263944
GGGAGGT248200.011.2667783
GCATAGG161550.011.08709436
TGGCATA162200.010.77832834
TAGGGGA262350.09.88053739
GGAGGTC283250.09.8567164
GTCACTC280350.09.7018858
AGCATAG90650.09.44715345
CGTAGGG89350.09.343137
GGTCACT285450.09.3393947
GGCGTAG91150.09.13390935
ACTCTCC296850.09.10956911
GCGTAGG91400.09.10892536
GCGTTCG25950.09.10399415
CGCGTTC27500.08.83530214
TCAGACG72400.08.8249072
Produced by FastQC (version 0.11.5)