FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47010071
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT534300.031.84409345
AGAACGA617200.027.57429144
AGACCGC632800.027.0405884
CAGACCG646500.026.5592943
ACCGCGT655750.026.0406536
GACCGCG660550.025.834375
CCGCGTT666250.025.6336357
CGCGTTC671500.025.4432728
AAGAACG676650.025.16450143
TCAGACC694700.024.930822
GCGTTCT693600.024.6455549
CTCAGAC730100.024.4151951
AATACGG700050.024.18215432
ATACGGA701200.024.12206533
TACGGAG700650.024.10596534
CAATACG711500.023.87939531
ACGGAGA736200.022.90525235
CGGAGAG737050.022.79025536
CCCAATA752000.022.71127729
CGTTCTC773600.022.32661410
Produced by FastQC (version 0.11.5)