FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47010071
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG566790.12056778216735729No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT504690.10735784678989319No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG502620.10691751561064436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG166200.015.37878845
ATAGGGG175050.014.72937538
GGCATAG177200.014.37311835
GGGAGGT280850.013.7102693
GAGCTTA191200.013.45003544
GTTGTCG21450.013.21647726
TGGCATA201700.012.93958734
GGAGGTC318400.012.1911164
CATAGGG226450.011.52527237
GGTCACT329900.011.3834587
GTCACTC349050.011.0160278
GCATAGG238650.010.88899936
TGGGAGG266800.010.3430042
TCTCCCC366950.010.22696713
TATCCTA74100.010.0208755
ATATAGC33300.09.9330596
TGTCCAA386300.09.80238826
CTCTGTC390550.09.73042823
GCTCTGT393500.09.63460922
AGCATAG120600.09.60803845
Produced by FastQC (version 0.11.5)