FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48649150
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT929890.19114208573017205No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG569200.11700101646174703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG23500.022.7850176
GACCGCG201650.021.4772665
GAACGAT202800.021.4550845
ACCGCGT203100.021.3457816
AGACCGC206800.020.9968934
CGCGTTC211650.020.4303918
CCGCGTT213150.020.3498867
GCCGGCT172250.019.7586561
CAGACCG224200.019.5379333
CCGGCTT203950.019.2495252
TTCACGC198650.018.7774337
TCACGCT199150.018.6173788
AACGCTA188800.017.7342623
ACGCTAC188250.017.6905424
TCAGACC252650.017.5871752
AGAACGA250400.017.41244344
GCGTTCT254200.017.143359
CGCTACC195100.017.01289225
GTCCGTT36350.016.8433821
CGGCTTC240500.016.8011723
Produced by FastQC (version 0.11.5)