FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46930516
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1660250.3537676849749532No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1198310.2553370604320651No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT526330.11215090837697161No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG496310.10575421757561754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC330650.025.468434
CAGACCG337850.025.0188673
GACCGCG347500.024.1234195
ACCGCGT354600.023.6785016
CCGCGTT366800.022.8970747
CGCGTTC369700.022.6931218
TCAGACC387950.022.2573572
GAACGAT379800.021.74724845
GCGTTCT394250.021.3142599
CTCAGAC436200.021.2275181
GCCGGCT327350.019.4746341
AATACGG431800.019.02821532
AGAACGA437650.018.90728444
CCGGCTT383650.018.8182352
ATACGGA438250.018.81490333
TACGGAG435500.018.81489234
CGTTCTC456750.018.8062710
CAATACG458450.018.11350331
TCACGCT382250.018.063458
CCTCTCA473050.018.05999819
Produced by FastQC (version 0.11.5)